NORTHLAND COMMUNITY & TECHNICAL COLLEGE

Page 112

A&P 2
Lab Manual

B) Microscopic Testes XS

Sketch a XS of the Testes, label and identify the following:

Seminiferous tubules, Epididymis, Interstitial cells

The testes are paired organs wrapped in a tough connective tissue sheath called the tunica albuginea. They are surrounded by the scrotal sac, which keeps the testes on the exterior of the body cavity, where the temperature is somewhat cooler. The testes are the site of spermatozoa production and this process must occur at about 35 degrees C. The scrotal sac is lined with a layer of muscle called the dartos muscle. It is composed of smooth muscle fibers that contract when the testes are cold, thus bringing them closer to the body. When the environment around the testes is warm, the dartos muscles relax and the testes descend from the body, thus becoming cooler.

Extensions of the tunica albuginea enter the testis, dividing it into a number of lobes, each of which houses one to four highly coiled seminiferous tubules, the sperm-forming factories. The seminiferous tubules of each lobe converge to empty the sperm into the epididymis, located on the exterior aspect of the testis. Lying between the seminiferous tubules and softly padded with connective tissue are the interstitial cells, which produce testosterone, the hormonal product of the testis.

Observe a slide of the testes in cross section (Figure 10.4). Numerous tubules are seen in cross section. These are the seminiferous tubules. The gametes or spermatozoa are produced in seminiferous tubules in the testis.

Find the clusters of cells that frequently appear as triangles in between the tubules. These are called interstitial cells. They produce the male sex hormone, testosterone. Examine the seminiferous tubules under high magnification. You should be able to see the outer row of cells called the spermatogonia. These cells reproduce by mitosis to produce primary spermatocytes. The primary spermatocytes undergo meiosis, or reduction division, to eventually produce the sex cells. The primary spermatocytes divide to form secondary spermatocytes which are found closer to the lumen. The secondary spermatocytes become spermatids. Spermatids lose their remaining cytoplasm and mature into spermatozoa. Sertoli cells assist in the movement of the primary spermatocytes.

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Figure 10.4
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Figure 10.5
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Figure 10.6
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Figure 10.7
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Figure 10.8
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