NORTHLAND COMMUNITY & TECHNICAL COLLEGE

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A&P 2
Lab Manual

2) OBSERVABLE HUMAN TRAITS

In the preceding problems, we examined several human traits that are fairly simple and that follow the Mendelian pattern of inheritance. Most of our traits are much more complex, involving many genes of interactions between genes. As an example, hair color is determined by at least four genes, each one coding for the production of melanin, a brown pigment. Because the effect of these genes is cumulative, hair color can range form (little melanin) to very dark brown (much melanin).

Clearly, human traits are most interesting to humans. A number of traits listed below exhibit Mendelian inheritance. For each, examine your phenotype and fill in Table 11-1. List your possible genotype(s) for each trait. When convenient, examine your parents’ phenotypes and attempt to determine your actual genotype.

1) Mid-digital hair (Figure 11-2a). Examine the joint of your fingers for the presence of hair, the dominant condition (MM, Mm). Complete absence of hair is due to the homozygous recessive condition (mm). You may need hand lens to determine your phenotype. Even the slightest amount of hair indicates the dominant condition.

2) Tongue rolling (Figure 11-2b). The ability to roll one’s tongue is due to dominant allele, T. The homozygous recessive condition, t, results in the inability to roll one’s tongue.

3) Widow’s peak (Figure 11-2c). Widow’s peak describes a distinct downward pint in the frontal hairline and is due to the dominant allele, W. The recessive allele, w, results in a continuous hairline. (Omit study of this trait if baldness is affecting the hairline.)

4) Earlobe attachment (Figure 11-2d). Most individuals have free earlobes (FF, Ff). Homozygous recessives (ff) have earlobes attached directly to the head.

5) Hitchhiker’s thumb (Figure 11-2e). Although considerable variation exists in this trait, we will consider those individuals who cannot extend their thumbs backwards 45 degrees to be carrying the dominant allele, H. Homozygous recessive persons (hh) can bend their thumbs at least 45 degrees, if not farther.

6) Relative finger length (Figure 11-2f). An interesting sex-influenced (not sex-linked) trait relates to the relative lengths of the index and ring finger. In males, the allele for a short (S) index finger is dominant. In females, it is recessive. In rare cases each hand may be different. If one or both index fingers are greater or equal to the length of the ring finger, the recessive genotype is present in males, and the dominant present in females.

Use this link to Print off a table
Table 11.1 Common Human Genetic Traits


Figure 11-2
Some Readily Observable Human Mendelian Traits


3) Blood Types

http://www.biology.arizona.edu/human_bio/problem_sets/blood_types/Intro.html

The Human Genetics Tutorial with problem solving exercises concerning the inheritance of the ABO blood group alleles has resulted in a steady stream of inquiries to the Biology Project from mothers, grandmothers, and children inquiring about the possible blood type of the father of a given child. Here is a typical inquiry:

"I have been reading your info about inheritance of blood types and I am getting very confused! I am trying to figure out what blood type the father of my son could have since my son and I are both type A+. Also, my brother is type 0 and my mom is A+. We can't find anything that explains how this can be. Could you please help??? --From a concerned Mom in Alberta, Canada"

Read through the explanations of the inheritance of blood types and Rh factors before attempting to answer the questions. A review of the tutorials of blood types problem 11 and problem 13 of the Monohybrid Cross Problem Set of the Mendelian Genetics might also be helpful.

Human ABO markers
Blood types and genotypes?
How are ABO alleles inherited by our children?
Rh factor

Problem set
Answer questions 1-6


4) DNA Testing

Every human has a unique DNA pattern, with the exception of identical twins. Half of an individual's DNA comes from the biological mother, and the other half comes from the biological father. A paternity test determines what half of the child's DNA came from the mother. The remaining half came from the true biological father (this is called obligate paternal DNA). If the obligate paternal DNA matches the alleged father beyond a minimum probability of 99.9%, then the tested man is the true father and an inclusion is reported. If there are at least two differences in the DNA pattern, then the alleged father is excluded as the biological father of the child.

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Figure 11.3

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Figure 11.4


Part 1 DNA Forensics I

http://www.biology.arizona.edu/human_bio/problem_sets/DNA_forensics_1/DNA_forensics.html

The purpose of this problem set is to present an introduction to the use of the Restriction Fragment Length Polymorphism (RFLP) method to characterize human DNA samples as applied in paternity analysis and sex crimes investigations. You will have the opportunity to interpret actual case results as might be produced by the FBI laboratory or a commercial, paternity-testing facility.

Answer questions 1-6

Instructions: The following problems have multiple choice answers. Correct answers are reinforced with a brief explanation. Incorrect answers are linked to tutorials to help solve the problem.

1. Southern hybridization technique
2. Paternity: problem one
3. Paternity: problem two
4. Rape investigation
5. VNTR: hyper-variable regions
6. Probability