NORTHLAND COMMUNITY & TECHNICAL COLLEGE

Page 127

A&P 2
Lab Manual

Part 2 DNA Forensics II

http://www.biology.arizona.edu/human_bio/problem_sets/DNA_forensics_2/DNA_forensics.html

The purpose of this problem set is to present an introduction to the use of DNA profile analysis to characterize human DNA samples as applied in paternity analysis and sex crimes investigations. You will have the opportunity to interpret actual case results as might be produced by the FBI laboratory or a commercial, paternity-testing facility.

Answer questions 1-10

Instructions: The following problems have multiple choice answers. Correct answers are reinforced with a brief explanation. Incorrect answers are linked to tutorials to help solve the problem.

1. HLA-DQ alpha
2. The variation of VNTRs
3. Methods of VNTR analysis
4. More methods of VNTR analysis
5. Alleles in a single-locus probe
6. Sources of DNA
7. Rape with two suspects
8. Another rape with two suspects
9. Reconstructing a missing mother's profile
10.Determining paternity


Part 3 DNA Forensics: Paternity

Shown to the right are DNA "fingerprints" from a new mother (M), her recently born child (C), and two possible fathers (F1 and F2). As is to be expected, the mother and the child share a few DNA bands since they also share 50% of their genes but they each have bands that are not represented among the bands of the other.

It is clear that the two possible fathers have dissimilar DNA banding patterns. The question is does one of the possible fathers have several bands similar to the child, which are not shared with either the mother or the other possible father. What do you think - was it (F1) or was it (F2)?

Answer questions 1-2

1) Who is the father?
2) Why do you think he is the father?

Part 4 DNA Patterns of Twins

Shown to the right are DNA banding patterns (restriction fragment length polymorphisms) from four sets of twins. If the band patterns are identical, then the twins should be identical (maternal) but if the band patterns of two twins are different, then the twins are non-identical (fraternal). Non-identical twins should share some of their DNA bands since they share 50% of the genes.

Answer questions 1-2

1) Which twins are identical?
2) Why do you think these are identical?