BIOLOGY 1111

Chapter 5

CELL MEMBRANE  

Northland Community & Technical College

Instructor Terry Wiseth

 

CELL MEMBRANE

cell membrane made up of:

1) Phospholipids

2) Sterols

3) Proteins

4) Glycoproteins

PHOSPHOLIPIDS

cell membrane - semi-permeable lipid bilayer

two layers of Phospholipids

hydrophilic head

hydrophobic acid tails

lipid bilayer minimizes the number of hydrophobic groups exposed to water

sealing behavior exhibited because the puncture is energetically unfavorable

    leaves too many hydrophobic groups exposed to the surrounding water

FLUID MOSAIC MODEL

membrane bilayer shows fluid behavior

molecules of the bilayer are in constant motion

membrane is composite of molecules allowing a “mosaic description”

cell survival depends on fluidity characteristics of the cell membrane

extracellular fluid temperature decreases cause a stiffening of the cell membrane

disrupts membrane protein function

yeast and bacteria cells under decreased temperatures rapidly synthesize unsaturated fatty acids

infusion of kinky lipids keep the membrane from stiffening up

STEROLS

common sterols of cell membranes

Cholesterol  (animal cells)

Phytosterols  (plant cells)

prevent packing of lipid cells in the cell membrane

GLYCOPROTEINS

proteins are embedded in the bilayer

glycoproteins - sugar-protein combinations

sugars extend out to the extracellular fluid 

ROUTES ACROSS CELL MEMBRANES

1) Diffusion / Osmosis

2) Protein Mediated Diffusion

3) Endocytosis and Exocytosis

DIFFUSION

diffusion process is a key factor in movement of substance across cell membranes and through the cytoplasm of the cell

movement of molecules from areas of high concentrations to areas of lower concentrations

net movement of molecules occurs down the concentration gradient

oxygen, carbon dioxide and small molecules with no charge

diffuse without assistance or consumption of energy

rate of diffusion is dependent on:

1) steepness of the concentration gradient

2) temperature

3) molecular size

4) electrical gradients

5) pressure gradients

OSMOSIS

movement of water across a semi-permeable membrane in response to solute concentration gradients

some small molecules (like water) pass readily through the cell membrane

glucose must be pumped across the membrane

water concentration gradient is influenced by the number of molecules of solutes that are present on both sides of the membrane

the direction in which water moves across the membrane is influenced by tonicity

TONICITY

relative concentrations of solutes in two fluids

water tends to move where solute concentrations are greatest

1) Isotonic=solute concentrations are equal

2) Hypotonic=least solute concentrations

3) Hypertonic=greatest solute concentrations

OSMOSIS

water molecules move from hypotonic solutions to hypertonic solutions

cells require built in mechanisms for adjusting to differences in tonicity between cytoplasm and their surroundings

without them cells will:

1) shrivel (crenation)

2) burst (lyse)

TURGOR PRESSURE

water moves into plant cells creating an internal pressure called turgor pressure

maintains soft parts of plant in an erect posture

soil must be hypotonic

plants will wilt if soil has too many solutes (hypertonic)

ex:  adding salty water

PROTEIN MEDIATED DIFFUSION

glucose, large water soluble molecules and charged ions

cannot diffuse freely across the cell membrane

A) Passive Transport

B) Active Transport

PASSIVE TRANSPORT

molecules diffuse through non-selective channel proteins

selective carrier proteins can move molecules of a solute across the cell membrane by binding and changing shape

passive transport occurs only down the concentration gradient

ACTIVE TRANSPORT

transport occurs when carrier proteins receive an energy boost from ATP

pump solutes across the cell membrane against the concentration gradient

ex:  calcium pump, sodium-potassium pump

cytoplasm concentrations of K+ increase and Na+ decrease

TYPES OF MEMBRANE PROTEINS

1) Channel Proteins

2) Active Transport Proteins

3) Receptor Proteins

4) Recognition Proteins

5) Adhesion Proteins

CHANNEL PROTEINS

Passive and do not require any energy investment by the cell

most remain open at all times

acts as a pore for water-soluble ions

may have molecular gates

ex:  nerve cells during nerve impulses

1) molecules only move down the concentration gradient

2) does not require any energy input from the cell

FACILITATED DIFFUSION

Does not require energy

Highly selective

Polar compounds such as sugars and amino acids

Diffusion is down the concentration gradient

ACTIVE TRANSPORT PROTEINS

require energy to transport molecules

1) molecules are pumped against their concentration gradient

2) requires an energy input from the cell

CARRIER PROTEINS

a change in shape allows bound substances to pass through the membrane

some may require an energy input to actively pump substances across the membrane

CONTRACTILE VACUOLES

paramecium live in freshwater

cytoplasm is hypertonic to the water the organism lives in

paramecium expel excess water influx by utilizing a contractile vacuole

RECEPTOR PROTEINS

have binding sites for hormones

ex:  hormone somatotropin binds to receptors which triggers enzymes to activate cell growth and division

RECOGNITION PROTEINS

act as “molecular fingerprints” on the cell surface

self recognition proteins identify cell type to other cells

white blood cells are able to recognize “self” from “non-self”

glycoproteins are extended into the extracellular fluid for easy access for recognition

ADHESION PROTEINS

connect cells together in a given tissue

glycoproteins connect neighboring cells

some may become cell junctions

ROUTES ACROSS CELL MEMBRANES

1) Diffusion / Osmosis

2) Protein Mediated Diffusion

3) Endocytosis and Exocytosis

EXOCYTOSIS AND ENDOCYTOSIS

move materials in bulk across cell membranes

EXOCYTOSIS

cytoplasmic vesicles fuse to the cell membrane and contents are released to the outside of the cell

ENDOCYTOSIS

1) Phagocytosis

2) Pinocytosis

region of the cell membrane sinks inward and balloons around substances, pinching itself off into a sealed vesicle which transports or stores the contents

PHAGOCYTOSIS

1) Phagocytosis “cell eater”

ex:  amoeba, white blood cells (macrophage)

lobes of cytoplasm wrap around the trapped item and seal together

newly formed vesicles fuse with lysosomes for digestion

PINOCYTOSIS

2) Pinocytosis “cell drinking”

ex:  intestinal cell taking up liquid droplets

 

END
 CHAPTER 5

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